Detecting Between-Pathway Model conservation across S. cerevisiae and S. pombe yeast species.
Malmer, Daniel A.
- The Between-Pathway Model (BPM) motif identifies pairs of fault-tolerant gene pathways within the yeast interactome. In BPMs, many gene pairs between the two pathways contain synthetic lethal interactions, meaning if you knock out one gene or the other only, the yeast lives, while knocking out both is lethal. This suggests that each pathway in a BPM compensates when an opposite pathway is suppress... read moreed, defective, or absent. Algorithms have been used to find suggested BPMs in the Saccharomyces cerevisiae and Schizosaccharomyces pombe species of yeast. Here we identify and apply a method for finding conservation of BPMs across these two species. By mapping orthologous genes between the two yeast species, we are able to identify BPM pathways which are highly conserved. Furthermore, with Gene Ontology (GO) enrichment, we can infer functional conservation as well. Given the large evolutionary distance between S. cerevisiae and S. pombe, a high rate of rewiring within the interactome is generally expected. However, in certain BPMs we found a large percentage of gene conservation and GO enrichment. Some of the pathways we identified were even found in the literature to be conserved across mouse and human. With this research, we hope to identify pathways which are evolutionarily conserved across S. cerevisiae and S. pombe, and encourage continued study of Between-Pathway Models.read less