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Background: Shigella dysenteriae serotype 1 (SD1) causes the most severe
form of epidemic bacillary dysentery. Quantitative proteome profiling of Shigella
dysenteriae serotype 1 (SD1) in vitro (derived from LB cell cultures) and in vivo
(derived from gnotobiotic piglets) was performed by 2D-LC-MS/MS and APEX, a
label-free computationally ... read moremodified spectral counting methodology.
Keywords: absolute protein expression, cytoplasmic membrane,
lipopolysaccharide, mass spectrometry, outer membrane, type III secretion system,
tricarboxylic acid, transmembrane domain.
Springer Open.read less
- Kuntumalla, Srilatha, Quanshun Zhang, John C. Braisted, Robert
D. Fleischmann, Scott N. Peterson, Arthur Donohue-Rolfe, Saul Tzipori, and Rembert
Pieper. "In vivo versus in vitro protein abundance analysis of Shigella
dysenteriaetype 1 reveals changes in the expression of proteins involved in
virulence, stress and energy metabolism." BMC Microbiology 11, no. 1 (12, 2011):