%0 PDF %T In vivo versus in vitro protein abundance analysis of Shigella dysenteriaetype 1 reveals changes in the expression of proteins involved in virulence, stress and energy metabolism. %A Kuntumalla, Srilatha.; Zhang, Quanshun.; Braisted, John C.; Fleischmann, Robert D.; Peterson, Scott N.; Donohue-Rolfe, Arthur.; Tzipori, Saul.; Pieper, Rembert. %D 2016-08-16T18:21:25.480Z %8 2016-08-16 %R http://localhost/files/9z903b410 %X Background: Shigella dysenteriae serotype 1 (SD1) causes the most severe form of epidemic bacillary dysentery. Quantitative proteome profiling of Shigella dysenteriae serotype 1 (SD1) in vitro (derived from LB cell cultures) and in vivo (derived from gnotobiotic piglets) was performed by 2D-LC-MS/MS and APEX, a label-free computationally modified spectral counting methodology.; Keywords: absolute protein expression, cytoplasmic membrane, lipopolysaccharide, mass spectrometry, outer membrane, type III secretion system, tricarboxylic acid, transmembrane domain.; Springer Open. %[ 2018-10-11 %9 Text %~ Tufts Digital Library %W Institution