Engineering Oral Formulation of Adenosine and Parabacteroides distasonis for Suppressing Colorectal Cancer
Inflammation in GI tract can cause many kinds of cancers, such as colorectal cancer
(CRC). As we can see from the previous research, adenosine is a famous kind of
anti-inflammation in GI tract, so increase of adenosine can suppress inflammation and
decrease the risk of CRC. P.dist (Parabacteroides distasonis) is a kind of anaerobic
bacteria in gastrointestinal tract. Studies have ... read morefound that P.dist bacteria is always
absent in CRC patients. Both live P.dist bacteria and P.dist bacteria lysate have the
function to suppress inflammation. The goal of this research is to investigate the oral
administration of adenosine and P.dist (either in live bacteria or bacteria lysate) to
suppress the inflammation and CRC. There are some barriers for oral delivery such as
fast half life of adenosine and intolerance of P.dist (both live or lysate), so I want
to develop an effective approach to deliver adenosine and P.dist orally. The first
chapter mainly focuses on developing an approach to adenosine encapsulation. In this
part I use wax, Eudragit S100, and Eudragit L100 to formulate the particle and calculate
encapsulation efficiency, stability and release kinetics for the particle in order to
show the effectiveness of encapsulation methods. The second chapter develops an approach
to encapsulate live P.dist or P.dist lysate. In this part, I use Eudragit FS30D to
formulate the particle. Since P.dist are obligate anaerobes, it is hard to imitate the
living environment in vitro and so, E.coli are used as the
Thesis (M.S.)--Tufts University, 2016.
Submitted to the Dept. of Chemical and Biological Engineering.
Advisor: Qiaobing Xu.
Committee: Jimmy Crott, and Ayse Asatekin.
Keyword: Biomedical engineering.read less