%0 PDF %T Engineering Oral Formulation of Adenosine and Parabacteroides distasonis for Suppressing Colorectal Cancer %A Ma, Qiannan. %8 2017-04-19 %R http://localhost/files/4t64h035n %X Abstract: Inflammation in GI tract can cause many kinds of cancers, such as colorectal cancer (CRC). As we can see from the previous research, adenosine is a famous kind of anti-inflammation in GI tract, so increase of adenosine can suppress inflammation and decrease the risk of CRC. P.dist (Parabacteroides distasonis) is a kind of anaerobic bacteria in gastrointestinal tract. Studies have found that P.dist bacteria is always absent in CRC patients. Both live P.dist bacteria and P.dist bacteria lysate have the function to suppress inflammation. The goal of this research is to investigate the oral administration of adenosine and P.dist (either in live bacteria or bacteria lysate) to suppress the inflammation and CRC. There are some barriers for oral delivery such as fast half life of adenosine and intolerance of P.dist (both live or lysate), so I want to develop an effective approach to deliver adenosine and P.dist orally. The first chapter mainly focuses on developing an approach to adenosine encapsulation. In this part I use wax, Eudragit S100, and Eudragit L100 to formulate the particle and calculate encapsulation efficiency, stability and release kinetics for the particle in order to show the effectiveness of encapsulation methods. The second chapter develops an approach to encapsulate live P.dist or P.dist lysate. In this part, I use Eudragit FS30D to formulate the particle. Since P.dist are obligate anaerobes, it is hard to imitate the living environment in vitro and so, E.coli are used as the model.; Thesis (M.S.)--Tufts University, 2016.; Submitted to the Dept. of Chemical and Biological Engineering.; Advisor: Qiaobing Xu.; Committee: Jimmy Crott, and Ayse Asatekin.; Keyword: Biomedical engineering. %[ 2022-10-12 %9 Text %~ Tufts Digital Library %W Institution