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Microbial chemotaxis is the directed movement of swimming cells toward
favorable chemical cues. In particular, chemotaxis plays a crucial role in marine
ecosystems by regulating biogeochemical processes. To untangle the web of microscale
chemotactic interactions, the response of diverse bacterial species to a range of
different chemical cues must be systematically screened across various chemical
... read more concentrations for multiple replicates.To aid in the screening process, we engineered a
rapid screening assay for bacterial chemotactic responses through the design and
fabrication of a multiplexed microfluidic device. The device uses serial dilution to
enable five simultaneous chemotaxis assays spanning five orders of magnitude in
chemoattractant concentration plus a control assay. Combined with automated microscopy
and further redesign, the device has the potential to reduce 960 individual chemotaxis
assays down to just 32, vastly improving the efficacy of microbial
screening.read less
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