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Abstract: Reliable
optical markers are key to timely detection of cervical precancer caused by high-risk
strains of human papillomavirus (HPV). Using a murine model, we acquired time-lapse
epifluorescence images of intrinsic NADH and FAD fluorescence in monolayer cultures of
dermal fibroblasts with and without the HPV-16 E7 gene. We measured
the resulting image sequences with 38 different ... read morespatiotemporal measurement
formulas, many of which use a novel software algorithm to estimate the fractal dimension
of irregularly shaped textures such as cell interiors. We found seventeen
intrinsic-fluorescence marker candidates for three experimental conditions: nine for
E7 expression, five for observed mitosis, and three for mitotic
abnormality. Each is statistically significant (p < 0.05) as well
as a uniquely significant marker of its condition, at least 20 times less likely to be
caused by chance than for the other two effects. We also identified markers most likely
to be robust to imperfect image focus.
Thesis
(M.S.)--Tufts University, 2011.
Submitted to the
Dept. of Biomedical Engineering.
Advisor: Irene
Georgakoudi.
Committee: Karl Munger, and Sergio
Fantini.
Keywords: Biophysics, Virology, and
Cellular biology.read less
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