%0 PDF %T Intrinsic-fluorescence markers reveal high-risk HPV infection and cervical precancer. %A MacDonald, Douglas. %8 2017-04-19 %R http://localhost/files/3t9462914 %X Abstract: Reliable optical markers are key to timely detection of cervical precancer caused by high-risk strains of human papillomavirus (HPV). Using a murine model, we acquired time-lapse epifluorescence images of intrinsic NADH and FAD fluorescence in monolayer cultures of dermal fibroblasts with and without the HPV-16 E7 gene. We measured the resulting image sequences with 38 different spatio­temporal measurement formulas, many of which use a novel software algorithm to estimate the fractal dimension of irregularly shaped textures such as cell interiors. We found seventeen intrinsic-fluorescence marker candidates for three experimental conditions: nine for E7 expression, five for observed mitosis, and three for mitotic abnormality. Each is statistically significant (p < 0.05) as well as a uniquely significant marker of its condition, at least 20 times less likely to be caused by chance than for the other two effects. We also identified markers most likely to be robust to imperfect image focus.; Thesis (M.S.)--Tufts University, 2011.; Submitted to the Dept. of Biomedical Engineering.; Advisor: Irene Georgakoudi.; Committee: Karl Munger, and Sergio Fantini.; Keywords: Biophysics, Virology, and Cellular biology. %[ 2022-10-12 %9 Text %~ Tufts Digital Library %W Institution