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Abstract: Under
homeostatic conditions, the commensal microbes of the microbiota protect themselves and
their host against enteric pathogens through colonization resistance. Colonization
resistance comprises of three key defense mechanisms: microbial release of
antimicrobials, crowding out through competition for nutrients and/or through the
microbial release of molecules to stimulate the hos... read moret immune response. When antibiotics
are applied to the microbiota there is a decrease in microbial diversity, weakening the
colonization resistance and leaving the host susceptible to pathogenic invasion,
infection, and disease. To assess the host and microbial activity under antibiotic
stress, preliminary metabolic profiles of differently antibiotic-conditioned mice were
generated using untargeted LC-MS/MS detection and a novel annotation pipeline. Analysis
of the metabolic profiles identified several metabolites that may reinforce colonization
resistance to deter pathogenic establishment in the gut during an antibiotic-stressed
state.
Thesis (M.S.)--Tufts University,
2017.
Submitted to the Dept. of Chemical and
Biological Engineering.
Advisor: Kyongbum
Lee.
Committee: Soha Hassoun, and Emmanuel
Tzanakakis.
Keywords: Biology, and
Microbiology.read less
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