| Description |
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Background: Although next generation sequencing (NGS) offers the
potential for studying virus populations in unprecedented depth, PCR error,
amplification bias and recombination during library construction have limited its use
to population sequencing and measurements of unlinked allele frequencies. Here we
report a method, termed ... read moreultrasensitive Single-Genome Sequencing (uSGS), for NGS
library construction and analysis that eliminates PCR errors and recombinants, and
generates single-genome sequences of the same quality as the "gold-standard" of HIV-1
single-genome sequencing assay but with more than 100-fold greater
depth.
Keywords: HIV, Single-genome sequencing, SGS, Primer ID, Supermajority
correction, Allele linkage, Targeted next-generation sequencing, NGS, Deep
sequencing, Minority variants, HIV drug resistance.
Springer Open.read less
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This object is in collection
| Citation |
- Boltz, Valerie, Jason Rausch, Wei Shao, Junko Hattori, Brian
Luke, Frank Maldarelli, John W. Mellors, Mary F. Kearney, and John M. Coffin.
"Ultrasensitive single-genome sequencing: accurate, targeted, next generation
sequencing of HIV-1 RNA." Retrovirology 13, no. 1 (12, 2016):
1-17.
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