Abstract: Currently, there is convincing evidence that the fibroblast-rich-barrier along the titanium surface plays a fundamental role in the establishment and maintenance of a proper soft tissue seal between the oral environment and the peri-implant bone. Titanium dental implants are known to be inert. Currently, a trend is to associate agents to obtain a bio-active titanium surface to improve ... read morefibroblast adhesion. In this pilot study, we developed and tested a bio-active titanium surface aimed at promoting in vitro, fibroblast attachment and proliferation. To this purpose, we created a new type of molecule made of a backbone of silk fibroin (SF) grafted with the RGDS and TiBP peptides. Our findings demonstrated that this new molecule had excellent capacities to adsorb onto titanium surfaces. Moreover, this new surface influenced the attachment and morphology of Swiss 3T3 fibroblasts. In general, an increase in cell number and growth were observed on bio-active surfaces (coated with SF-RGD/TiBP) compared to bio-inactive machined titanium surfaces.
Thesis (M.S.)--Tufts University, 2012.
Submitted to the Dept. of Periodontology.
Advisors: terrence griffin, and Christophe Egles.
Committee: Rory O'Neill, and Driss Zoukhri.
Keywords: Dentistry, and Cellular biology.read less