Mutagenic inverted repeat assisted genome engineering (MIRAGE).

Nair, Nikhil U.

Zhao, Huimin (Professor)

2009.

Description
  • Here we describe a one-step method to create precise modifications in the genome of Saccharomyces cerevisiae as a tool for synthetic biology, metabolic engineering, systems biology and genetic studies. Through homologous recombination, a mutagenesis cassette containing an inverted repeat of selection marker(s) is integrated into the genome. Due to its inherent instability in genomic DNA, the inver... read more
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