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The role of membrane potential in adipose-derived stem cell (ASC) patterning has yet to be elucidated. To assess the effects of membrane potential modulation on human ASC behavior, ASCs were cultured in 2D treated and with membrane potential-altering drugs such as ivermectin. Additionally, to assess the combined effects of membrane potential modulation in a three-dimensional microenvironment, ASCs ... read morewere also cultured on silk fibroin sponges and treated with ivermectin. While some experiments showed ivermectin induce an increase in ASC proliferation, others did not show the same effect. Future experiments can be undertaken to evaluate the modulation of membrane potential in ASCs including use of other electroceutical drugs, assessing combined effects of growth factors, and performing experiments in media with defined chloride levels.read less
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