Bioavailability of Ellagic Acid, Urolithin A and Urolithin B in Human Plasma after Pomegranate Juice or Extract Consumption.
Pomegranate products have been reported to have many beneficial effects including
anti-oxidant, anti-inflammatory, and anti-proliferative properties. These health
benefits are more likely to be attributed to the metabolites found after absorption
rather than the intact components of pomegranate. To investigate the bioavailability of
Ellagic Acid (EA), Urolithin-A (Uro-A), and Urolithin ... read moreB (Uro-B), 12 healthy volunteers
were administrated with 2 doses of pomegranate juice (PJ trial, ~800mg polyphenol/dose)
or pomegranate extract (PE trial, ~689mg polyphenol/dose), and blood samples were
collected at various time points for up to 12.5 hours after dosage. Protein
precipitation by acetonitrile in an acidic environment was used for plasma extraction.
Free and conjugated metabolites were both analyzed by comparing incubation and
non-incubation with β-deglucuronidase. Because matrix effect varied among
different individuals and could not be completely eliminated, matrix-matched calibration
curve was adopted for analytical quantification. In volunteer no.3, free EA was detected
in both PE and PJ trials, fast absorbed and quickly eliminated with Tmax of ~1hr and AUC
of ~57 (ng·h)/ml after administration. Cmax for EA in PE and PJ trials were
different: 25.0ng/ml in PE trial vs. 15.2ng/ml in PJ trial. For Uro-A, neither free nor
glucuronide conjugated Uro-A were detectable in PE trial, but high concentrations of
Uro-A glucuronide were detected in PJ trial. Tmax for Uro-A would occur at least 12.5
hours later after consumption. Uro-B were undetectable in both trials. The evaluation of
the pharmacokinetic profiles of target analytes in other subjects is still in
Thesis (M.S.)--Tufts University, 2016.
Submitted to the Dept. of Pharmacology & Experimental Therapeutics.
Advisor: DAVID GREENBLATT.
Committee: MARGERY BEINFELD.
Keyword: Pharmacology.read less