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Kaposi's
sarcoma-associated herpesvirus, the etiologic agent of Kaposi's sarcoma and other
aggressive AIDS-associated malignancies, encodes over 90 genes, most of which are
expressed only during the lytic replication cycle. The role of many of the KSHV lytic
proteins in the KSHV replication cycle remains unknown, and many proteins are annotated
based on known functions of homologs in other ... read moreherpesviruses. Here I investigate the role
of the previously uncharacterized KSHV lytic protein ORF42, a presumed tegument protein.
I find that ORF42 is dispensable for reactivation from latency, but is required for
efficient production of viral particles. Like its alpha- and beta-herpesviral homologs,
ORF42 is a late protein that accumulates in the viral particles. However, unlike its
homologs, ORF42 is required for efficient accumulation of late viral proteins and may
potentiate post-transcriptional stages of gene expression. These results demonstrate
that ORF42 has an important role in KSHV replication and may contribute to shaping viral
gene expression.
Thesis (Ph.D.)--Tufts University,
2019.
Submitted to the Dept. of
Biochemistry.
Advisor: Marta
Gaglia.
Committee: Katya Heldwein, Claire Moore, and
Ralph Isberg.
Keywords: Virology, and
Biology.read less
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