Design and Optimization of a System for Automated Culture Medium Exchange in a Petri Dish.
Yan, Luqi.
2017-04-20T14:00:58.905Z
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Abstract: To culture a sample, one method is to put an experimental specimen into a culture medium and to wait for it to grow. Some samples must be cultured for specific requirements in biological experiments. Scientists need to change the culture medium during some experiments. There are some automated pipetting systems and perfusion systems that can be used to exchange culture medium. However, a... read morell current products have some limitations. At present, there is no appropriate method or device to automatically exchange culture medium in a petri dish for some specific samples, such as tadpoles. The major goal of this research was to design and demonstrate a device that can automatically exchange the culture medium in a petri dish. The automatic exchange device would have following advantages: flexible exchange settings, easy operation, no risk of dehydration, high exchange efficiency and low cost. This device would be a cost effective solution for automating tedious routine lab work, and may greatly promote new developments in biology. The device designed in this thesis mainly included two parts: an emptying system and a filling system. A sample device was manufactured to examine the filling system design and test the efficiency of the emptying system using a petri dish as the culture container. Two basic models and a series of adjustments were investigated for use in the emptying system. In order to explore the most efficient model, finite element analysis was performed to simulate the fluid flow in the petri dish, and simulation results were compared to experimental data.
Thesis (M.S.)--Tufts University, 2015.
Submitted to the Dept. of Mechanical Engineering.
Advisor: William Messner.
Committee: Michael Zimmerman, and Masoud Sanayei.
Keyword: Mechanical engineering.read less - ID:
- vd66wb19d
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