Design and Optimization of a System for Automated Culture Medium Exchange in a Petri Dish.
Yan, Luqi.
2015
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Abstract: To culture a
sample, one method is to put an experimental specimen into a culture medium and to wait
for it to grow. Some samples must be cultured for specific requirements in biological
experiments. Scientists need to change the culture medium during some experiments. There
are some automated pipetting systems and perfusion systems that can be used to exchange
culture medium. However, ... read moreall current products have some limitations. At present, there
is no appropriate method or device to automatically exchange culture medium in a petri
dish for some specific samples, such as tadpoles. The major goal of this research was to
design and demonstrate a device that can automatically exchange the culture medium in a
petri dish. The automatic exchange device would have following advantages: flexible
exchange settings, easy operation, no risk of dehydration, high exchange efficiency and
low cost. This device would be a cost effective solution for automating tedious routine
lab work, and may greatly promote new developments in biology. The device designed in
this thesis mainly included two parts: an emptying system and a filling system. A sample
device was manufactured to examine the filling system design and test the efficiency of
the emptying system using a petri dish as the culture container. Two basic models and a
series of adjustments were investigated for use in the emptying system. In order to
explore the most efficient model, finite element analysis was performed to simulate the
fluid flow in the petri dish, and simulation results were compared to experimental
data.
Thesis (M.S.)--Tufts University, 2015.
Submitted to the Dept. of Mechanical Engineering.
Advisor: William Messner.
Committee: Michael Zimmerman, and Masoud Sanayei.
Keyword: Mechanical engineering.read less - ID:
- vd66wb19d
- Component ID:
- tufts:21571
- To Cite:
- TARC Citation Guide EndNote
