The Effect of 2% Chlorhexidine Gluconate on Microleakage of Total-Etch, Self-Etch and Selective Etch Adhesives.
Aldeeb, Laila.
2013
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Abstract: Objectives: The primary purpose of this in vitro study was to compare the marginal leakage between total-etch, self-etch, and selective-etch adhesive systems, both with and without applying 2% chlorhexidine. Then, the marginal leakage between the chlorhexidine groups and the nonchlorhexidine groups were compared. Materials and Methods: 105 extracted intact human molars were mounted in ... read moreautopolymerizing acrylic resin. Standardized Class II preparations were prepared on the occluso-proximal surfaces. Then the teeth were randomized in seven groups: group 1: Total-etch adhesive (37% phosphoric acid and bond with Scotchbond Universal); group 2: Self-etch adhesive (Scotchbond Universal); group 3: Selective-etch adhesive (etching enamel with 37% phosphoric acid and bond with Scotchbond Universal); group 4: Total-etch adhesive with 2% chlorhexidine disinfectant (Consepsis); group 5: Self-etch adhesive then 2% chlorhexidine disinfectant; group 6: 2% chlorhexidine disinfectant then bonded with self-etch adhesive; group 7: Selective-etch adhesive with 2% chlorhexidine disinfectant. All the teeth were restored with Filtek Supreme composite, then thermocycled (5ºC and 55ºC) for 2000 cycles. Next, the teeth were immersed in a solution of 50 wt% ammoniacal silver nitrate for 24 hours, followed by 8 hours in a photo-developing solution. Finally, the teeth were sectioned and measured by two raters from the occlusal and cervical parts. The specimens were analyzed using a stereomicroscope and scored according to the degree of dye penetration along the dentin walls; score 0: no marginal leakage; score 1: silver nitrate (SN) penetrates up to 2 half the length of the gingival wall; score 2: SN penetrates beyond half the gingival wall length; score 3: SN penetrates along the gingival and axial walls toward the pulp. Statistical significance was assessed via Generalized Estimating Equations (GEE). A Bonferroni correction was used with the new cutoff: 0.05/13, which is approximately 0.004. A cross tabulation between the first rater and the second rater was made and the Kappa statistic was computed. SAS version 9.2 and SPSS 19 were used in the analysis. Results: At least 76.7% of the specimens in groups 1, 3, 4 and 7 had 0 score of microleakage. On the other hand, 30% or less of the specimens from groups 2, 5 and 6 had 0 score of microleakage. Upon comparing between the non-chlorhexidine groups, there was a statistically significant difference between group 1 and group 2 (p-value<0.0001), and between group 2 and group 3 (p-value<0.0001). When comparing the chlorhexidine groups to each other, group 4 showed a significant difference with group 5 (p-value=0.002), as well as with group 6 (p-value=0.001). There was no significant difference between group 5 and group 6 (p-value=0.681). However, there was a significant difference when comparing group 5 to group 7 (p-value<0.0001), and group 6 to group 7 (p-value<0.0001). When the groups were compared based on the type of adhesive system used, the results revealed no statistically significant difference among them. Conclusions: Total-etch and selective-etch adhesive systems have superior results over self-etch adhesive systems, with or without the use of chlorhexidine. Chlorhexidine did not adversely affect the bond of either of the adhesive systems. Applying chlorhexidine, either before the self-etch adhesive or after, did not have have a statistically significant effect on the microleakage scores.
Thesis (M.S.)--Tufts University, 2013.
Submitted to the Dept. of Other.
Advisor: David Bardwell.
Committee: Matthew Finkelman, Hiroshi Hirayama, and Ronald Perry.
Keyword: Dentistry.read less - ID:
- tx31qv99k
- Component ID:
- tufts:20203
- To Cite:
- TARC Citation Guide EndNote
