Background: We used a PCR-based approach to study the prevalence of
genetic sequences related to a gammaretrovirus, xenotropic murine leukemia
virus-related virus, XMRV, in human prostate cancer. This virus has been identified
in the US in prostate cancer patients and in those with chronic fatigue syndrome.
However, with the exception of ... read moretwo patients in Germany, XMRV has not been identified
in prostate cancer tissue in Europe. Most putative associations of new or old human
retroviruses with diseases have turned out to be due to contamination. We have looked
for XMRV sequences in DNA extracted from formalin-fixed paraffin- embedded prostate
tissues. To control for contamination, PCR assays to detect either mouse
mitochondrial DNA (mtDNA) or intracisternal A particle (IAP) long terminal repeat DNA
were run on all samples, owing to their very high copy number in mouse
Robinson, Mark, Otto W. Erlwein, Steve Kaye, Jonathan Weber,
Oya Cingoz, Anup Patel, Marjorie M. Walker, Wun-Jae Kim, Mongkol Uiprasertkul, John
M. Coffin, and Myra O. McClure. "Mouse DNA contamination in human tissue tested for
XMRV." Retrovirology 7, no. 1 (12, 2010): 1-6.