Inquiry into the Hyperinfectivity of Vibrio cholerae.
host passage Vibrio cholerae enters a hyperinfectious phase. This transient phase of
increased infectivity likely plays an important role in the rapid spread of cholera
during outbreaks. As a complex phenotype, hyperinfectivity should be viewed as a
collection of host-induced phenotypes that improve the fitness of V. cholerae during
subsequent infection. To date, neither ... read moreregulators of the hyperinfectivity nor important
characteristics of the phase have been determined. We took a three-pronged approach to
studying hyperinfectivity: genetic, proteomic and phenotypic. We used Tn-seq to examine
the genes important for hyperinfectivity in the two main animal models; we determined
rice water stool-associated proteome, and we determined stress resistance associated
with host-passaged V. cholerae. Our data indicate that host-passaged V. cholerae reduces
population diversity in the secondary infection, undergoes changes in susceptibility to
stress and contains a unique set of membrane proteins not expressed during growth in
Thesis (Ph.D.)--Tufts University, 2014.
Submitted to the Dept. of Molecular Microbiology.
Advisor: Andrew Camilli.
Committee: Ralph Isberg, Michael Malamy, Joan Mecsas, and Jay Zhu.
Keyword: Microbiology.read less