Assessing the Degree of Candida Albicans Adhesion to Two Types of Orthotic Appliance Materials. An In Vitro Study.
Abstract: ABSTRACT Purpose: The aim of the study was to assess the amount of C. albicans adhered to two types of orthotic appliance materials before and after modification with the use of a self-bonding polymer, (SBP, KISS-COTE). In addition, the effectiveness of multiple cleaning methods for the removal of C.albicans of those materials was also evaluated. Materials and Methods: Two types of mater... read moreials were examined, acrylic and vinyl materials. Both materials were modified or not with KISS-COTE polymer. Twenty-four samples of each subgroup were tested after being incubated in the C.albicans suspension. Samples were stained with Gram's crystal violet, examined under a light microscope, and digitally photographed. The percentages of adhered C.albicans were calculated by using Image J software. As a second phase, specimens were cleaned by four cleaning methods: water, ultrasonic cleaner, toothbrush and Polident tablets. Percentages of the remaining C.albicans were analyzed, as described above. Results: The comparison of percentages of C.albicans on acrylic without KISS-COTE subgroup (mean=94.52) and vinyl without KISS-COTE subgroup (mean=93.80) was not statistically significant (P=0.11). The comparison between the groups of KISS-COTE modified surfaces was statistically significant, with vinyl group exhibiting more adherence (vinyl with KISS-COTE median=81.37, acrylic with KISS-COTE median=59.9) (P<0.001). There was a statistical significant difference when compared acrylic with KISS-COTE group and vinyl with KISS-COTE group to their controls (P<0.001). All cleaning methods removed C.albicans from the appliances when compared to the control group (water) (P<0.008). Brushing showed the best degree of removal compared to the other cleaning methods. Conclusions: The amount of C. albicans adhering to acrylic material without KISS-COTE compared to vinyl material without KISS-COTE showed no significant difference. However, there was a significant difference between the two materials with modified surfaces. Brushing method was the most effective in removing C. albicans from all tested materials.
Thesis (M.S.)--Tufts University, 2015.
Submitted to the Dept. of Other.
Advisor: Driss Zoukhri.
Committee: Tofool Alghanem, Noshir Mehta, George Maloney, and Mathew Finkeman.
Keywords: Dentistry, and Microbiology.read less