Observing single enzyme molecules interconvert between activity states upon heating.

Rojek, Marcin J.

Walt, David R.


  • In this paper, we demonstrate that single enzyme molecules of β-galactosidase interconvert between different activity states upon exposure to short pulses of heat. We show that these changes in activity are the result of different enzyme conformations. Hundreds of single β-galactosidase molecules are trapped in femtoliter reaction chambers and the individual enzymes are subjected to short heating ... read more
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  • Rojek MJ, Walt DR (2014) Observing Single Enzyme Molecules Interconvert between Activity States upon Heating. PLoS ONE 9(1): e86224. doi:10.1371/journal.pone.0086224.
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