Observing single enzyme molecules interconvert between activity states upon heating.

Rojek, Marcin J.

Walt, David R.

2014

Description
  • In this paper, we demonstrate that single enzyme molecules of β-galactosidase interconvert between different activity states upon exposure to short pulses of heat. We show that these changes in activity are the result of different enzyme conformations. Hundreds of single β-galactosidase molecules are trapped in femtoliter reaction chambers and the individual enzymes are subjected to short heating ... read more
This object is in collection Creator department Subject Permanent URL Citation
  • Rojek MJ, Walt DR (2014) Observing Single Enzyme Molecules Interconvert between Activity States upon Heating. PLoS ONE 9(1): e86224. doi:10.1371/journal.pone.0086224.
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rf55zm304
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