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Abstract: Fermentation has been established as a viable source for the commercial production of erythromycin. Despite efforts in metabolic engineering for strain improvement, the optimization of the fermentation process remains critical in the production and productivity of erythromycin. The newly postulated methodology of the Design of Dynamic Experiments (DoDE) facilitates the optimization of th... read moreis process by examining the proper design of a set of experiments to achieve a data-driven optimization. The Design of Dynamic Experiments approach optimizes a process with respect to time varying conditions. A simulated environment is used in which a foreign protein mathematical model is modified for erythromycin production. Simulated experiments investigate optimum substrate and inducer feed flow rate profiles with respect to erythromycin production. Optimal conditions were identified using the DoDE that provides a 98.5% increase of erythromycin production when compared to the classical DOE approach. An economically viable set of experiments is recommended to use in experiments.
Thesis (M.S.)--Tufts University, 2011.
Submitted to the Dept. of Chemical and Biological Engineering.
Advisor: Christos Georgakis.
Committee: Blaine Pfeifer, and Christopher Loose.
Keyword: Chemical Engineering.read less
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