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Abstract: Fermentation has been established as a viable source for the
commercial production of erythromycin. Despite efforts in metabolic engineering for strain
improvement, the optimization of the fermentation process remains critical in the
production and productivity of erythromycin. The newly postulated methodology of the Design
of Dynamic Experiments (DoDE) facilitates the optimization o... read moref this process by examining the
proper design of a set of experiments to achieve a data-driven optimization. The Design of
Dynamic Experiments approach optimizes a process with respect to time varying conditions. A
simulated environment is used in which a foreign protein mathematical model is modified for
erythromycin production. Simulated experiments investigate optimum substrate and inducer
feed flow rate profiles with respect to erythromycin production. Optimal conditions were
identified using the DoDE that provides a 98.5% increase of erythromycin production when
compared to the classical DOE approach. An economically viable set of experiments is
recommended to use in experiments.
Thesis (M.S.)--Tufts University, 2011.
Submitted to the Dept. of Chemical and Biological
Engineering.
Advisor: Christos Georgakis.
Committee: Blaine Pfeifer, and Christopher Loose.
Keyword: Chemical Engineering.read less
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