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Abstract: Fermentation
has been established as a viable source for the commercial production of erythromycin.
Despite efforts in metabolic engineering for strain improvement, the optimization of the
fermentation process remains critical in the production and productivity of
erythromycin. The newly postulated methodology of the Design of Dynamic Experiments
(DoDE) facilitates the optimization ... read moreof this process by examining the proper design of a
set of experiments to achieve a data-driven optimization. The Design of Dynamic
Experiments approach optimizes a process with respect to time varying conditions. A
simulated environment is used in which a foreign protein mathematical model is modified
for erythromycin production. Simulated experiments investigate optimum substrate and
inducer feed flow rate profiles with respect to erythromycin production. Optimal
conditions were identified using the DoDE that provides a 98.5% increase of erythromycin
production when compared to the classical DOE approach. An economically viable set of
experiments is recommended to use in
experiments.
Thesis (M.S.)--Tufts University,
2011.
Submitted to the Dept. of Chemical and
Biological Engineering.
Advisor: Christos
Georgakis.
Committee: Blaine Pfeifer, and
Christopher Loose.
Keyword: Chemical
Engineering.read less
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