Salivary Biomarkers of Periodontal Disease in Smoker and Non-Smoker Periodontitis Patients.
Abstract: Background: Smoking alters the host response, including vascular function, neutrophil/monocyte activities, antibody production, and cytokine and inflammatory mediator release. Interleukin-1 beta (IL-1β), osteoprotegerin (OPG) and matrix metalloproteinase 8 (MMP8) are involved in three aspect of periodontal disease: inflammation, collagen degradation and bone resorption. Therefore, ... read moremeasurement of salivary concentration of these biomarkers could be used as a reliable indicator of periodontal disease activity. Hence, the objectives of this study were to determine and compare the salivary concentrations of IL-1β, MMP8 and OPG in smokers versus non-smokers with periodontal disease as well as healthy individuals and determine if they correlate with clinical features of periodontal disease. Methods: A total of 87 Participants (30 periodontaly healthy (H), 28 non-smoker individuals with periodontitis (P), 29 smoker individuals with periodontitis (SP)) formed the study group. After periodontal examination including probing depth (PD), clinical attachment level (CAL) and bleeding, saliva samples were collected and the amounts of IL-1β, MMP8 and OPG were quantified using enzyme-linked immunosorbent assays. Results: Bleeding was found to be highest in periodontitis and smoker periodontitis groups (2.62±0.48, 1.82± 0.47 respectively), with a statistically significant results (p = 0.001). IL-1β and MMP8 levels in saliva were found to be highest in smokers with periodontitis (7.25± 7.14, 3494.80±1092.89 respectively), OPG level in saliva were found to be highest in periodontitis (744.79±1617.68) without statistical significance (p = 0.374). Week correlations were found between level of OPG with PD and CAL that was statistically significant results (p = 0.019, and 0.012 respectively). Conclusion: bleeding level are suggested to be affected by smoking habits. Longitudinal, prospective studies will help to verify the observations of the present study. Further research in this direction could reveal reliable markers to forecast the progress of periodontal disease in smoker subjects.
Thesis (M.S.)--Tufts University, 2014.
Submitted to the Dept. of Periodontology.
Advisor: Driss Zoukhri.
Committee: Paul Stark, Athena Papas, Rory O'Neill, and Yong Hur.
Keywords: Dentistry, and Health sciences.read less
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