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Abstract: DNA
sequencing of phage display products presents a combination of two technologies capable
of providing a high throughput means of screening protein-protein and protein-substrate
interactions. Phage display presents a system for the controlled interrogation of
molecular interactions at the expressed protein level, yet uses the encoding DNA as the
analyte. Recent advances in DNA seq... read moreuencing technologies have rendered this technology a
tractable readout for a variety of applications, including phage display. The method
described circumvents the need for any infection of phagemid into bacteria with the
intent of removing amplification as a source of bias. In order to understand the utility
of this technology for screening and discovery of amino acid motifs involved in
extracellular matrix adhesion, highly diverse phage libraries were screened against
silk, collagen, and polystyrene substrates. Products were PCR amplified and prepared for
Illumina sequencing. Recovered sequences were translated in silico to peptide sequences
and aligned to known extracellular matrix protein sequences to identify regions of
substrate interaction.
Thesis (M.S.)--Tufts
University, 2012.
Submitted to the Dept. of
Biomedical Engineering.
Advisors: David Kaplan, and
Nathaniel Cosper.
Committee: Hyumin Yi, and Qiaobing
Xu.
Keyword: Biomedical
engineering.read less
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