| Description |
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Background: The throughput of next-generation sequencing machines has
increased dramatically over the last few years; yet the cost and time for library
preparation have not changed proportionally, thus representing the main bottleneck
for sequencing large numbers of samples. Here we present an economical,
high-throughput library preparation ... read moremethod for the Illumina platform, comprising a
96-well based method for DNA isolation for yeast cells, a low-cost DNA shearing
alternative, and adapter ligation using heat inactivation of enzymes instead of bead
cleanups.
Keywords: Next-generation sequencing, High throughput, DNA isolation,
Yeast, DNA fragmentation, Heat inactivation, Recombination,
Aneuploidy.
Springer Open.read less
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This object is in collection
| Citation |
- Wilkening, Stefan, Manu M. Tekkedil, Gen Lin, Emilie S.
Fritsch, Wu Wei, Julien Gagneur, David W. Lazinski, Andrew Camilli, and Lars M.
Steinmetz. "Genotyping 1000 yeast strains by next-generation sequencing." BMC
Genomics 14, no. 1 (12, 2013): 1-10.
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