Background: The throughput of next-generation sequencing machines has
increased dramatically over the last few years; yet the cost and time for library
preparation have not changed proportionally, thus representing the main bottleneck
for sequencing large numbers of samples. Here we present an economical,
high-throughput library preparati... read moreon method for the Illumina platform, comprising a
96-well based method for DNA isolation for yeast cells, a low-cost DNA shearing
alternative, and adapter ligation using heat inactivation of enzymes instead of bead
Keywords: Next-generation sequencing, High throughput, DNA isolation,
Yeast, DNA fragmentation, Heat inactivation, Recombination,
Wilkening, Stefan, Manu M. Tekkedil, Gen Lin, Emilie S.
Fritsch, Wu Wei, Julien Gagneur, David W. Lazinski, Andrew Camilli, and Lars M.
Steinmetz. "Genotyping 1000 yeast strains by next-generation sequencing." BMC
Genomics 14, no. 1 (12, 2013): 1-10.