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Background: Despite a number of different transgenes that can mediate
DNA deletion in the developing lens, each has unique features that can make a
given transgenic line more or less appropriate for particular studies. The purpose
of this work encompasses both a review of transgenes that lead to the expression
of Cre recombinase in the lens and a comparative analysis of currently available
... read moretransgenic lines with a particular emphasis on the Le-Cre and P0-3.9GFPCre lines
that can mediate DNA deletion in the lens placode. Although both of these
transgenes are driven by elements of the Pax6 P0 promoter, the Le-Cre transgene
consistently leads to ocular abnormalities in homozygous state and can lead to
ocular defects on some genetic backgrounds when hemizygous.
Keywords: Cre recombinase, Lens development, Transgenic
mice.
Springer Open.read less
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