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Abstract: A silk-based
hydrogel system was explored to evaluate the effects of substrate stiffness and cyclic
stretch on human mesenchymal stem cell (hMSCs) differentiation. The hydrogel fibers were
highly tunable, elastic and supported the survival and growth of encapsulated hMSCs. The
hMSCs in silk hydrogel fibers at 50 kPa and 150 kPa both transferred from a multipotent
stem cell state to ... read morea pre-osteoblast/adipocyte state in static culture. With cyclic
stretching, osteo-differentiation was enhanced, while adipo-differentiation was
suppressed. A new method to generate a tunable, anisotropic 3D porous scaffold with
branching networks to enhance vascularization in vitro and direct anastomosis in vivo
was also developed. Branched channels were generated in the silk-based scaffolds with
proper endothelialization. Silk fibers in the scaffold aligned with a specific direction
and achieved anisotropic diffusion of molecules with different sizes. These new
silk-based tissue engineered constructs can integrate physical and chemical cues for
desired stem cell differentiation to improve tissue
regeneration.
Thesis (M.S.)--Tufts University,
2018.
Submitted to the Dept. of Biomedical
Engineering.
Advisors: Lauren Black III, and David
Kaplan.
Committee: Emmanuel
Tzanakakis.
Keyword: Biomedical
engineering.read less
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