Multiplexed bioelectronic platforms for probing electrophysiology in cardiac and neural tissue models
Rao, Akshita A.
2021
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Bioelectronic recording devices open new avenues for long-term electrophysiology by enabling detection and measurement of signals produced by cardiomyocytes and neurons. Electrical measurements that provide the shape and duration of action potentials give information regarding the density, type, and metabolic state of ion channels. Current devices are limited by challenges, including immune responses ... read moreto implanted devices, mechanical mismatch between rigid electronics and tissue, and limited quantities of electrodes. This project introduces a 32-channel planar multielectrode array (MEA) and a flexible, macroporous array of electrodes (FMEA) and demonstrates their ability to collect multiplexed electrophysiological signals. From previous work, it is known that contracting cardiomyocytes and neurons fire action potentials which can be measured by an extracellular sensor in close proximity to the cell membrane. These noninvasive, long-term, real-time measurements enable electrical synchrony monitoring and tissue maturation tracking. Fabrication protocols were established to achieve a planar and flexible bioelectronic and PCB interface. The planar MEA devices were fabricated with >85% functioning devices, and the FMEA design yielded with >60% functioning devices. Further studies of cardiac and neural activity through electrophysiology readings can better inform pharmaceutical and therapeutic developments through disease modeling and drug screening applications.
Advisor: Dr. Brian Timkoread less - ID:
- ms35tp87b
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