Cardiometabolic risk factors, iron status and hepcidin in obese individuals undergoing weight loss.
Hepcidin is a hormone that regulates cellular iron export. Obesity is characterized by a
state of low-grade chronic inflammation and elevated hepcidin both in circulation and
adipose tissue. Chronic overexpression of hepcidin in obesity may lead to iron status
impairment. The effect of weight loss (WL) through calorie restriction (CR) on hepcidin
and iron status in obese... read moreadults is not known. Hepcidin is expressed in adipose tissue
(AT) in obesity, but the effect of WL on AT hepcidin expression has not been determined.
Objective: We investigated the effect of WL through CR on systemic inflammation,
hepcidin and iron status, and WL-induced changes on AT hepcidin and macrophage marker
expression. Design: A CR intervention trial was conducted in 26 obese adults. Serum
hepcidin, C-reactive protein (CRP), iron status, and AT expression of hepcidin,
adipokines, and expression of macrophage markers were assessed before and after WL.
Results: AT hepcidin was inversely correlated with iron status at baseline (p<0.05).
WL correlated with decreased serum hepcidin in subjects who had at least 5% WL
(p<0.05, N=14). Change in waist circumference also correlated with decreased serum
hepcidin and CRP (p<0.05). There was no significant change in iron status with WL. AT
expression of CD68, a macrophage marker, was highly correlated with AT hepcidin
expression before (p<0.001) and after (p<0.0001) WL. CD14 and TNFá,
correlated with hepcidin at baseline only. Conclusion: Our data show a significant
association between WL through CR and decrease in serum hepcidin and inflammation, but
not iron status. However, there was an inverse association between AT hepcidin and iron
status, as well as a tight association between AT macrophages, AT inflammation and
hepcidin. Further research is needed to determine the role of inflammatory dysregulation
of AT in obesity-related iron status
Thesis (Ph.D.)--Tufts University, 2013.
Submitted to the Dept. of Biochemical and Molecular Nutrition.
Advisor: Simin Meydani.
Committee: Edward Saltzman, Davidson Hamer, and Caroline Apovian.
Keywords: Nutrition, Immunology, and Gerontology.read less