Device for Wireless Delivery of Electrical Stimulation for Sterile Culture of Cardiomyocytes.
cardiomyocyte growth in vitro requires electrical and mechanical stimulation; however,
current electrical stimulation methods are non-sterile, requiring wires to enter the
incubator. This research describes the development of a wireless electrical stimulation
system for the growth of cardiomyocytes in a sterile environment. This consisted of two
components: a radiofrequency (... read moreRF) transmitter, an RF receiver and rectifier, and an
electrode. An externally triggered function generator was set up to deliver short pulses
(~5ms) of RF radiation. Two iterations of a design containing a Petri dish with a
combination of an antenna, interdigitated electrode, and rectifier circuit were used to
recieve and rectify the pulses for electrical stimulation. The initial design was tested
with HL-1 cells, an immortalized cardiomyocyte cell line to verify the effectiveness of
the electrode design. Under varied wireless stimulation conditions, pacing of the cells
was achieved at stimulation frequencies between 1-2Hz. Benchtop tests of the final
design, a fully integrated version of the first, prove the working principles of the
device. Future cells studies will verify the stimulation capabilities of the new design
and cell viability after long term
Thesis (M.S.)--Tufts University, 2015.
Submitted to the Dept. of Biomedical Engineering.
Advisor: Fiorenzo Omenetto.
Committee: Lauren Black, and Michael Levin.
Keyword: Biomedical engineering.read less