Comparison of Candida Albicans Adhesion to Various Denture Base Materials.
Abstract: Objectives: Denture stomatitis is a common fungal infection, affecting upto 76% of removable denture patients and is often associated with Candida Albicans (CA) biofilms. A wide range of materials with various processing techniques are used for denture bases. This research examines the adhesion of CA to the surface of various denture base materials and its relation to surface roughness. ... read moreMethods: Rectangular samples of (15x5x2mm)(n=15) were fabricated for seven different denture base materials (1-Avadent CAD/CAM, 2-Eclipse, 3-SR Ivocap High Impact (SRIHI), 4-Clear Ivocap (CI) 5-Lucitone FRS flexible (LFRS), 6-Nature-CRYL Pour (NCPGC), 7-DC acrylic S.P. (DCSP). After polishing, surface roughness (Ra) values were measured using a Mitutoyo profilometer. All samples were incubated with CA suspension (SC5314) for 1 hour at 37°C. The serial dilutions of the original suspension (106 cells/ml) were then plated with the suspension that was removed from the material (Plate 20 ul of 10-2 and 10-3 dilutions), spread on several glucose agar plates and aerobically incubated at 37°C for 48 h. Adherence was calculated by the total amount of CFU (Colony Forming Unit) in each plate including the amounts bound to pieces, and % binding . Statistical analyses were performed using Kruskal-Wallis tests and Spearman Correlation analysis. Results: Median CA adhesion values ranged from 0.0 (Avadent) to 8.0 (Eclipse) (p<0.02). Overall, Eclipse, CI (4.3) and SRIHI (1.4) showed more adhesion than LFRS (0.3), DCSP (0.1), NCPGC (0.2) and Avadent (0) groups. Ra values also varied: FRS (0.7) and Eclipse (0.05) showed rougher surfaces than CI (0.03), SRIHI (0.03), NCPGC (0.12) and Avadent (0.03) groups (p<0.02). Significant correlations were observed between CA and Ra as well. Conclusions: Denture base materials differ in surface roughness values and CA adhesion; smoother surfaces had less CA adhesion.
Thesis (M.S.)--Tufts University, 2014.
Submitted to the Dept. of Posthodontics.
Advisor: Ali Muftu.
Committee: Carol Kumamoto, Matthew Finkelman, and Amit Sachdeo.
Keyword: Dentistry.read less