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CAG repeat sequences are found throughout the human genome and are hotspots of mutation due to their tendency to form non-B secondary structures. My work investigates the mechanisms behind the elevated CAG repeat fragility phenotypes of two RNA processing mutants: thp2∆ and trf4∆. While previous literature suggests that R-loops are the cause of genomic instability associated with these mutants, my ... read morework suggests that impaired RPA loading may be more important for fragility at expanded CAG repeats in these mutants. I also detail the construction of an inducible promoter system, which will allow the Freudenreich lab to investigate the effect of transcription on fragility in the future.read less
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