Recombinant fibrous protein production and applications
Abstract: Fibrous proteins are natural polymers with repetitive units that
form higher order structures upon supramolecular interactions. These polymers are most
frequently built from structural building blocks owing to the unique mechanical properties
that stem from the hierarchical alignment of the smaller units. The facile production of
tunable recombinant fibrous proteins as alternatives t... read moreo their natural protein counterparts
and their manipulation for different applications will promote the progress of biomedical
research. Collagen is the most abundant fibrous protein in all metazoan life and it is
found in a variety of tissues in the body, maintaining the structural integrity while
interacting with cells and extracellular molecules. This thesis will focus on collagen and
its interactions with cell receptors, as well as collagen diseases. The study of the
biological consequences of mutations within the integrin binding region of collagen using
the bacterial collagen system increases our understanding of collagen interactions. This
thesis improves our molecular understanding of the effects such Gly missense mutations have
on integrin binding. The results may have relevance for clarifying the molecular basis of
to the subset of OI Gly missense mutations that are within or nearby the integrin binding
sequence. This application of the bacterial collagen system illustrates the influence of
substitutions and exposes novel outcomes for the interplay between collagen mutations and
Thesis (Ph.D.)--Tufts University, 2016.
Submitted to the Dept. of Chemistry.
Advisor: David Kaplan.
Committee: Joshua Kritzer, Krishna Kumar, and Matthew Shoulders.
Keywords: Biomedical engineering, Biochemistry, and Chemistry.read less