| Description |
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Background: The glutamate synthase operon (gltBDF) contributes to one of
the two main pathways of ammonia assimilation in Escherichia coli. Of the seven
most-global regulators, together affecting expression of about half of all E. coli
genes, two were previously shown to exert direct, positive control on gltBDF
transcription: Lrp and IHF. ... read moreThe involvement of Lrp is unusual in two respects: first,
it is insensitive to the usual coregulator leucine, and second, Lrp binds more than
150 bp upstream of the transcription starting point. There was indirect evidence for
involvement of a third global regulator, Crp. Given the physiological importance of
gltBDF, and the potential opportunity to learn about integration of global regulatory
signals, a combination of in vivo and in vitro approaches was used to investigate the
involvement of additional regulatory proteins, and to determine their relative
binding positions and potential interactions with one another and with RNA polymerase
(RNAP).
Springer Open.read less
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| Citation |
- Paul, Ligi, Pankaj K. Mishra, Robert M. Blumenthal, and Rowena
G. Matthews. "Integration of regulatory signals through involvement of multiple
global regulators: control of the Escherichia coli gltBDF operon by Lrp, IHF, Crp,
and ArgR." BMC Microbiology 7, no. 1 (12, 2007): 1-17.
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