| Description |
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Background: Quantitative analysis of transcriptional regulation of genes
is a prerequisite for a better understanding of the molecular mechanisms of action of
different radiation qualities such as photon, proton or carbon ion irradiation.
Microarrays and real-time quantitative RT-PCR (qRT-PCR) are considered the two
cornerstones of gene ... read moreexpression analysis. In interpreting these results it is
critical to normalize the expression levels of the target genes by that of
appropriately selected endogenous control genes (ECGs) or housekeeping genes. We
sought to systematically investigate common ECG candidates for their stability after
different radiation modalities in different human cell lines by qRT-PCR. We aimed to
identify the most robust set of ECGs or housekeeping genes for transcriptional
analysis in irradiation studies.
Keywords: Endogenous control genes, Internal control genes, qRT-PCR,
Photon, Proton, Carbon-ion, Tumour cells, A431, A549 and BxPC3.
Springer Open.read less
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| Citation |
- Sharungbam, Geeta, Christian Schwager, Sara Chiblak, Stephan
Brons, Lynn Hlatky, Thomas Haberer, Jürgen Debus, and Amir Abdollahi. "Identification
of stable endogenous control genes for transcriptional profiling of photon, proton
and carbon-ion irradiated cells." Radiation Oncology 7, no. 1 (12, 2012):
1-12.
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