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Abstract: A. C. Figure 13. A. Configuration of media reservoir made up of a 30mL syringe barrel with a rubber stopper plugging up its open two. The two syringes piercing through the rubber stopper are connected to two male luer lock to barb connections which connect to tubes that lead out to a gas exchange and media flow in port. The female barb on the bottom of the syringe leads to the bioreactor... read morechamber (Note; during testing/imaging phenol red free media was used). B. 3DKUBETM chamber with polystyrene bored out and cover glass glued on using Loctite adhesive (gray ring around imaging interface). C. Perfusion flow circuit showing entire configuration from chamber to pump to media reservoir plus connections.
Thesis (M.S.)--Tufts University, 2012.
Submitted to the Dept. of Biomedical Engineering.
Advisor: David Kaplan.
Committee: Irene Georgakoudi, and Gary Leisk.
Keyword: Biomedical engineering.read less
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