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Abstract: A. C.
Figure 13. A. Configuration of media reservoir made up of a 30mL syringe barrel with a
rubber stopper plugging up its open two. The two syringes piercing through the rubber
stopper are connected to two male luer lock to barb connections which connect to tubes
that lead out to a gas exchange and media flow in port. The female barb on the bottom of
the syringe leads to the ... read morebioreactor chamber (Note; during testing/imaging phenol red
free media was used). B. 3DKUBETM chamber with polystyrene bored
out and cover glass glued on using Loctite adhesive (gray ring around imaging
interface). C. Perfusion flow circuit showing entire configuration from chamber to pump
to media reservoir plus connections.
Thesis
(M.S.)--Tufts University, 2012.
Submitted to the
Dept. of Biomedical Engineering.
Advisor: David
Kaplan.
Committee: Irene Georgakoudi, and Gary
Leisk.
Keyword: Biomedical
engineering.read less
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