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Abstract: Obesity is
a rising issue, especially in the United States, that can lead to heart problems, type
II diabetes, and respiratory problems. Since the 1970s, obesity rates in the United
States have more than doubled in adults and children. Recent evidence suggests that
exposure to certain chemicals, termed "obesogens", in utero may alter metabolic
processes, predisposing individuals to ... read moregain weight. The goal of this thesis is to
develop a three-dimensional platform that is able to model the effects of obesogens in
vitro. Human embryonic-derived stem cells in three-dimensional collagen embedded silk
scaffolds were exposed to three different obesogens. The exposed models had an increase
in accumulation of triglycerides and expression of adipogenic genes (PLIN1, PPARy, and
FABP4) compared to equivalent cultures with no obesogen exposures. These results
demonstrate that we were able to successfully develop a representative model of in utero
obesogen exposure.
Thesis (M.S.)--Tufts University,
2016.
Submitted to the Dept. of Biomedical
Engineering.
Advisor: David
Kaplan.
Committee: Irene Georgakoudi, and Kyongbum
Lee.
Keyword: Biomedical
engineering.read less
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