Identification of Pathogenic Lymphocyte Subsets in Ankylosing Spondylitis.
- Killer immunoglobulin-like receptors (KIRs) may play a role in the pathogenesis of ankylosing spondylitis (AS). KIR3DL2 binds to HLA-B27 homodimers and genetic associations with AS have been reported for KIR3DS1/DL1 and KIR2DL5. KIR expression is not limited to Natural Killer (NK) cells and has been demonstrated for other innate and adaptive lymphocyte subsets as well. We hypothesize that the ... read morepreviously described expansion of KIR3DL2 positive CD4+ T cells expressing the pathogenic cytokine IL-17A in HLA-B27+ individuals might be only one of many examples for the disease-promoting modulation of lymphocyte function by AS-associated KIRs in HLA-B27+ individuals. Distinct KIR expression profiles might identify pathogenic lymphocyte subsets in HLA-B27+ patients with AS. A 17-color flow cytometry panel was developed that distinguishes 8 lymphocyte subsets in human PBMCs stimulated with PMA/Ionomycin and permits the analysis of multiple KIRs and cytokines in a single sample. This panel will be used to analyze samples from HLA-B27+ and HLA-B27- healthy individuals in order to examine any correlations between HLA-B27 and KIR expression.read less