%0 PDF %T Characterization of MT3-MMP Production by Embryonic Tendon Progenitor Cells during Development and the Influence of Substrate Elastic Modulus on MT3-MMP Production. %A Bench, Steven. %8 2017-04-20 %R http://localhost/files/9593v629h %X Abstract: Tendons are frequently injured, but current treatment strategies aimed at repairing or regenerating tendon have remain insufficient due to a limited understanding of which factors regulate cell behavior, matrix assembly, and mechanical properties. By elucidating these factors, we will gain valuable insights into how tendons heal and develop, which may allow for improved tendon tissue engineering strategies. Tendon healing and development are characterized by substantial extracellular matrix turnover, which is thought to be driven by matrix metalloproteinases, including MT3-MMP. MT3-MMP has been shown to be present in embryonic tendon, but only at late stages of development by Western blot. MT3-MMP has been implicated as a possible regulator of tissue development, but its role in tendon development has not yet been revealed. Here, a detailed description of MT3-MMP production during embryonic tendon development is presented, and substrate elastic modulus is explored as a potential regulator of MT3-MMP production. Immunofluorescent staining and image analysis were utilized to measure MT3-MMP protein distribution during tendon development. Embryonic tendon progenitor cells were cultured on polyacrylamide gels of different elastic moduli to determine the effect of substrate elastic modulus of MT3-MMP production. Our results showed that MT3-MMP protein is present throughout tendon development, and protein production decreases in response to increased substrate elastic modulus, possibly through the actin cytoskeleton.; Thesis (M.S.)--Tufts University, 2014.; Submitted to the Dept. of Biomedical Engineering.; Advisor: Catherine Kuo.; Committee: Mitch McVey, and Qiaobing Xu.; Keyword: Biomedical engineering. %[ 2022-10-12 %9 Text %~ Tufts Digital Library %W Institution