Backgound: Shiga toxin 2 (Stx2), one of two Stx liberated by
Stx-producing Escherichia coli, is composed of an A subunit monomer and a B subunit
pentamer, and is directly linked with hemolytic uremic syndrome in children. The
pentameric B subunit binds to its cell surface receptor Gb3 for toxin
internalization, and the A subunit follows i... read morentracellular retrograde transport to the
cytosol where its RNA N-glycosidase activity (RNA-NGA) shuts down the protein
synthesis, and leads to cell death. The present study investigated the ability of 19
Stx2 A subunit-specific human monoclonal antibodies (HuMAbs) to neutralize the
RNA-NGA, and the association this neutralizing activity with protection of HeLa cells
and mice against Stx2-induced death.
Jeong, Kwang-il, Susan Chapman-Bonofiglio, Pradeep Singh,
Jongo Lee, Saul Tzipori, and Abhineet S. Sheoran. "In vitro and in vivo protective
efficacies of antibodies that neutralize the RNA N-glycosidase activity of Shiga
toxin 2." BMC Immunology 11, no. 1 (12, 2010): 1-9.