Bioskiving: Tendon-derived Scaffolds for Biomedical Applications.
Alberti, Kyle.
2015
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Abstract: The work contained herein is the development of a method to directly
utilize the intricate and proven structures contained within tendon for biomedical
applications ― a technique called Bioskiving. The hypothesize is that this sectioning-based
technique will allow for maintenance of these structures while creating thin sheets that
are flexible and can be formed into desired shapes. T... read morehis fabrication process is applied to
tendon, in order to capitalize on the mechanical and biological properties of the
hierarchical and well-ordered collagen structures found within. This work progresses
through the development of the processing technique and creation of scaffolds, which
involves: decellularization, sectioning, and stacking and rolling to create two- and
three-dimensional scaffolds. The mechanical properties of the material are then
characterized, and tuned using crosslinking to achieve a 20-fold increase in mechanical
strength and transverse isotropy. The biological properties of the material are also
characterized, including the degradation in vitro and in vivo, and the interaction of the
material with platelets. Lastly, one potential application of constructs comprised of the
material is explored, peripheral nerve repair. The material's potential for nerve repair is
evaluated in vitro using Schwann cells and chick dorsal root ganglia explants, and in vivo
using a rat sciatic nerve defect model. The tendon-derived material proves to be a suitable
substrate for promoting peripheral nerve repair, and is biocompatible and biodegradable.
The mechanical properties can be tuned and the geometry of structures created be altered,
allowing others to find use for it in a number of biomedical applications.
Thesis (Ph.D.)--Tufts University, 2015.
Submitted to the Dept. of Biomedical Engineering.
Advisor: Qiaobing Xu.
Committee: David Kaplan, Lauren Black, Barry Trimmer, and Bing Xu.
Keyword: Biomedical engineering.read less - ID:
- 8p58pq576
- Component ID:
- tufts:21346
- To Cite:
- DCA Citation Guide EndNote
