Bioskiving: Tendon-derived Scaffolds for Biomedical Applications.
Abstract: The work
contained herein is the development of a method to directly utilize the intricate and
proven structures contained within tendon for biomedical applications ― a
technique called Bioskiving. The hypothesize is that this sectioning-based technique
will allow for maintenance of these structures while creating thin sheets that are
flexible and can be formed into desired shapes. ... read moreThis fabrication process is applied to
tendon, in order to capitalize on the mechanical and biological properties of the
hierarchical and well-ordered collagen structures found within. This work progresses
through the development of the processing technique and creation of scaffolds, which
involves: decellularization, sectioning, and stacking and rolling to create two- and
three-dimensional scaffolds. The mechanical properties of the material are then
characterized, and tuned using crosslinking to achieve a 20-fold increase in mechanical
strength and transverse isotropy. The biological properties of the material are also
characterized, including the degradation in vitro and in vivo, and the interaction of
the material with platelets. Lastly, one potential application of constructs comprised
of the material is explored, peripheral nerve repair. The material's potential for nerve
repair is evaluated in vitro using Schwann cells and chick dorsal root ganglia explants,
and in vivo using a rat sciatic nerve defect model. The tendon-derived material proves
to be a suitable substrate for promoting peripheral nerve repair, and is biocompatible
and biodegradable. The mechanical properties can be tuned and the geometry of structures
created be altered, allowing others to find use for it in a number of biomedical
Thesis (Ph.D.)--Tufts University, 2015.
Submitted to the Dept. of Biomedical Engineering.
Advisor: Qiaobing Xu.
Committee: David Kaplan, Lauren Black, Barry Trimmer, and Bing Xu.
Keyword: Biomedical engineering.read less