Effect of Coating Titanium Implants with Silk on Osseointegration.
Abstract: Available in-vitro data suggest that surface modeling of inert titanium with silk enhances its interaction with the biological environment. Furthermore, incorporating arginine-glycine-aspartic acid (RGD) peptides into silk might stimulate the adhesion of fibroblasts leading to an accelerated osseointegration. The purpose of this study was to test the effect of coating implants with ... read moreRGD-decorated silk on osseointegration in an in-vivo parallel design, randomized controlled trial in an animal model. A total of 18 titanium implants were used. Each animal received 3 coated (test) and 3 uncoated (control) implants placed in their tibiae. Bone samples were collected at 2, 4, and 6 weeks post-implant placement. The percentage of bone-to-implant contact (BIC) was assessed histologically. Different inflammatory cells were identified around each implant to assess the inflammatory response. The BIC in the test group was higher than the control group (82% vs. 62%, p = 0.083) at 2 weeks but lower at 4 weeks (39% vs. 57%, p = 0.275) and 6 weeks (32% vs. 34%, p=1.000), but none of these differences were statistically significant. The number of lymphocytes was higher in the test group at 2, 4, and 6 weeks (p=0.025, 0.034, and 0.025, respectively). Lymphocytes, MN Giant cells and macrophages were also higher in number with statistical significance in the test group across the different time points. Our data suggest that coating implants with RGD decorated silk does not improve the short-term BIC and initiates a higher inflammatory response. However, further studies with larger sample sizes and modified coating protocol, are needed to be performed.
Thesis (M.S.)--Tufts University, 2013.
Submitted to the Dept. of Periodontology.
Advisor: Driss Zoukhri.
Committee: Paul Stark, Hans-peter Weber, Gerard Kugel, and Natalie Jeong.
Keywords: Biology, and Biomedical engineering.read less