Monitoring Nerve Growth Factor Release From Silk Films For Peripheral Nerve Regeneration.
- Peripheral nerve damage is a severe problem that can lead to pain or even loss of limb function. Current clinical methods of treating peripheral nerve damage are not satisfactory. To have more control over regenerating peripheral nerves, researchers are creating nerve conduits. Much research has been done on creating nerve conduits, but poor material choice and lack of growth promoters, such as ne... read morerve growth factor (NGF), have inhibited regeneration. Recently, nerve conduits have been made from silk fibroin. There are many methods of incorporating NGF into a nerve conduit. To achieve higher rates of NGF release, the study discussed in this paper analyzed different methods of incorporating NGF into silk fibroin. In this study, a system that can be applied to create nerve conduits for peripheral nerve regeneration was developed by characterizing the release of NGF from a 2D model of a nerve conduit. Silk films were used to model the nerve conduit release characteristics to provide a simpler system for analysis. There were three approaches of incorporating NGF in the silk. In the first model, the NGF was mixed with aqueous silk solution. In the second model, a layer of NGF was dried on top of the silk film. In the third model, layers on NGF were entrapped between layers of silk. All of the methods yielded low release rates of NGF.read less