Histological Study of Pulp Response to Different Durations of Topical Triple Antibiotic Medicament.
Abstract: Objectives: The aim of this study was to investigate the impact of topical triple antibiotics application duration (3, 5, and 7 days) on disinfection of intentionally infected ferrets' canine teeth by histological examination of pulp response. Methods: Four ferret canine teeth in each group were intentionally infected under general anesthesia with Enterococcus fecalis for 6 weeks. Teeth ... read morewere assigned into four groups: control (no antibiotics); 3 days of triple antibiotic application; 5 days of triple antibiotic application; and 7 days of triple antibiotic application. Topical triple antibiotics ointment (metronidazole, ciprofloxacin, and minocycline) was applied on all teeth except the control group, which were extracted. All treated teeth were extracted 4 weeks following antibiotics removal. Tooth samples were prepared for histologic assessment of inflammatory cell response intensity and type, tissue necrosis, and bacterial presence. Periodontal ligament (PDL) thickness was assessed using initial, post-infection, and post-treatment radiographs. Results: Data were analyzed with the Generalized Estimating Equations. Control group showed statistically significantly higher inflammatory cell response intensity (p = 0.0125) and an acute type of inflammatory cells (p <0.001) compared to 3-days, 5-days, and 7-days groups. In contrast, there were no statistically significant differences between 3-days, 5-days, and 7-days groups in terms of inflammatory cell response intensity and type. There was no statistically significant difference in tissue necrosis and PDL thickness between any of the study groups. Conclusions: We can conclude that application of topical triple antibiotics on pulpally involved ferrets' teeth show a tendency to promote healing by decreasing the inflammation intensity and formation of fibrous pulp tissue. There was no significant difference between the longer and shorter treatment groups, which suggests that shorter application duration may be as suitable as the longer duration in treating pulpally involved teeth.
Thesis (M.S.)--Tufts University, 2014.
Submitted to the Dept. of Pediatric Dentistry.
Advisor: Driss Zoukhri.
Committee: Paul Stark, Cheen Loo, and Addy Alt-Holland.
Keyword: Dentistry.read less