Singlet Oxygen Mediated & Amplified Biosensing with Conjugated Polymers
Sarrafpour, Syena
2012
- This project combines both a chemical amplification as well as an optical amplification through the binding of singlet oxygen (1O2). When the photosensitizer, eosin 5-isothiocyanate, is bound to NeutrAvidin labeled biotinylated microspheres, 1O2 is generated; the singlet oxygen then reacts with the quenching conjugated acenes on the thin film to restore the conjugated polymer's fluorescence. Prior ... read moreto any 1O2 reaction with the acenes, there is an overlap between the poly (9, 9-di-n-dodecylfluorenyl-2, 7-diyl)'s (PF) emission and 5, 12-bis(4-methoxyphenyl)tetracene's (TET) absorption which allows for the PF's excited state to be transferred to TET. As singlet oxygen binds to TET, thermodynamically stable endoperoxides are formed, halting any quenching of PF's fluorescence. Therefore, the fluorescence shifts from the TET, at 510nm, to the conjugated polymer at 420 nm. These experiments demonstrated singlet oxygen, produced by the eosin 5-isothiocyanate/NeutrAvidin/biotinylated microspheres, mediated amplification of fluorescent signals through the ratiometric shift of the TET's fluorescence to that of the PF. This project is unique in its use of multiplicative amplification through wavelength ratiometric signals and singlet oxygen photosensitization without the use of enzymes.read less
- ID:
- 6q182x56n
- Component ID:
- tufts:UA005.015.015.00001
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