Improving adenine base editing precision by enlarging the recognition domain of CRISPR-Cas9.

Gao, Shuliang

Weng, Benson

Wich, Douglas

Power, Liam

Chen, Mengting

Guan, Huiwen

Ye, Zhongfeng

Xu, Chutian

Xu, Qiaobing

2025

open in viewer
Description
  • Domain expansion contributes to diversification of RNA-guidedendonucleases including Cas9. However, it remains unclear how REC domain expansion could benefit Cas9. In this study, we identify an insertion spot that is compatible with large REC insertion and succeeds in enlarging the noncatalytic REC domain of Streptococcus pyogenes Cas9. The natural-evolutionlike giant SpCas9 (GS-Cas9) is created ... read more
This object is in collection Creator department Subject Permanent URL Citation
ID:
5h73q942b
To Cite:
TARC Citation Guide    EndNote
Usage:
Detailed Rights
DOI:
https://doi.org/10.1038/s41467-025-57154-5