Protein and Virus Encapsulation for Delivery.
Zhou, Chensheng.
2013
-
Abstract: This thesis presents encapsulation techniques for biomedical
applications, including enzyme encapsulation and immobilization for correcting enzyme
deficiency, and vaccinia virus formulation for vaccinate wild animals. The first chapter
describes a formulation strategy that protects therapeutic enzymes by encapsulation and
immobilization them into a hydrogel. Enzyme deficiency related... read moregenetic disorder diseases
usually develop mildly but can cause vital damage when left untreated. Enzyme replacement
therapy, which artificially supplies a replacement of the defected enzyme, was first
demonstrated in 1970s.[1] It is an effective, cost effective and safe way to control these
diseases. In this research, we are figuring out a way to make the therapeutic enzyme more
compatible with in vivo environment, so they can last longer. Using enzyme encapsulation
and immobilization strategies, the enzyme activity can be preserved for longer time as well
as escaping from immune response. The second chapter is about engineering a bait-vaccine
oral delivery system, for controlling Lyme disease. Vaccinate the bacteria reservoir animal
is one of the approaches to control this infectious disease. We were developing a
formulation procedure for the vaccinia virus and antigen protein enteric delivery. Since
the vaccine will be distributed to the wild, there is additional concern about avoiding
random infections. We have adapted and invented several methods including wet formulation,
semi-dry formulation and dry formulation. Our goal was to engineer a reliable enteric
formulation for vaccinate wild animals.
Thesis (M.S.)--Tufts University, 2013.
Submitted to the Dept. of Biomedical Engineering.
Advisors: Qiaobing Xu, and Linden Hu.
Committee: Qiaobing Xu, Linden Hu, and Lauren Black.
Keywords: Biomedical engineering, and Pharmaceutical sciences.read less - ID:
- 4q77g377x
- Component ID:
- tufts:22054
- To Cite:
- DCA Citation Guide EndNote
