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Abstract: Obesity promotes the development of a variety of diseases including type 2 diabetes, cardiovascular disease, fatty liver, and cancer. As obesity is characterized by a chronic state of low-grade inflammation distinguished by immune cell infiltration into the adipose tissue, a model demonstrating this inflammatory state is needed to better understand the disease itself as well as how it af... read morefects different patients. Utilizing a 3D tissue-engineered in vitro adipose construct integrating a 3D silk fibroin porous scaffold and the multiple cell types found in adipose tissue, an induced inflammatory model was created by the addition of THP-1 derived macrophages. The model demonstrated increased cell proliferation, higher rates of lipolysis, and an upregulation of leptin production in a patient specific manner. The results demonstrate the successful development of an inflammatory state and highlight the patient specific response to inflammatory conditions.
Thesis (M.S.)--Tufts University, 2017.
Submitted to the Dept. of Biomedical Engineering.
Advisor: David Kaplan.
Committee: Lauren Black, and Kyongbum Lee.
Keyword: Biomedical engineering.read less
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