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Abstract: We have
proposed a method using the primer exchange reaction (PER) in which allows synthesis of
single stranded DNA using a catalytic hairpin to achieve highly multiplex resolution
imaging. PER cascades are an ideal candidate in amplifying signals with in vitro
extended probes in a programmable fashion. In parallel, we have studied the mechanical
properties and contributing factors ... read moreof different hydrogel composition in the context of
expansion microscopy (ExM). ExM enables imaging of cells and tissues with nanoscale
precision.
Thesis (M.S.)--Tufts University,
2018.
Submitted to the Dept. of Biomedical
Engineering.
Advisors: Yu Wang, and George
Church.
Committee: Yu Wang, Xiaocheng Jiang, and
Mark Cronin-Golomb.
Keyword: Biomedical
engineering.read less
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