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Abstract: The
ever-evolving nature of influenza virus strains continues to be a cause for concern in
preventing the next influenza pandemic or epidemic and combating resistance. In this
work, the mechanism of action of a novel anti-influenza liposome formulation is
explored. Since the liposome is designed to bind to influenza hemagglutinin receptor,
interaction of the virus and liposome was ... read moreinvestigated by size analysis and quantified
by surface plasmon resonance, which demonstrated that binding between the two can be
characterized by a dissociation constant (Kd) in the nanomolar range. Sialylated
liposomes were found to inhibit the infection of 103 plaque forming units (PFU) on MDCK
and human airway epithelial cells by 70-90% at nanomolar concentrations when pre-mixed
with virus, but were less effective when given prophylactically or post-infection. Thus,
binding of sialylated liposome to virus seems to be a prerequisite for preventing
infection, and consequently, for disrupting the kinetics of viral
replication.
Thesis (M.S.)--Tufts University,
2012.
Submitted to the Dept. of Biomedical
Engineering.
Advisors: James Comolli, and Qiaobing
Xu.
Committee: Lauren Black
III.
Keyword: Biomedical
engineering.read less
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